Localization and characterization of Xylella fastidiosa haemagglutinin adhesins

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Localization and characterization of Xylella fastidiosa haemagglutinin adhesins.

Xylella fastidiosa is a gram-negative, xylem-inhabiting, plant-pathogenic bacterium responsible for several important diseases including Pierce's disease (PD) of grapevines. The bacteria form biofilms in grapevine xylem that contribute to the occlusion of the xylem vessels. X. fastidiosa haemagglutinin (HA) proteins are large afimbrial adhesins that have been shown to be crucial for biofilm for...

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Characterization of a Diffusible Signaling Factor from Xylella fastidiosa

UNLABELLED Cell-cell signaling in Xylella fastidiosa has been implicated in the coordination of traits enabling colonization in plant hosts as well as insect vectors. This cell density-dependent signaling has been attributed to a diffusible signaling factor (DSF) produced by the DSF synthase RpfF. DSF produced by related bacterial species are unsaturated fatty acids, but that of X. fastidiosa w...

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Interaction between Xylella Fastidiosa and Curtobacterium Flaccumfaciens

Xylella fastidiosa (Xf) is a fastidious gram-negative, xylem-limited bacterium that causes diseases in many crops of economic importance, such as grape (Pierce’s disease), almond, peach, coffee, plum and citrus. Xf can infect all known Citrus sinensis cultivars and causes Citrus variegated chlorosis (CVC). One major endophyte isolated from CVC-asymptomatic plants is the bacterium Curtobacterium...

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EFFECTS OF FIMBRIAL (FIMA, FIMF) AND AFIMBRIAL (XADA, HXFB) ADHESINS ON THE ADHESION OF XYLELLA FASTIDIOSA TO SURFACES Project Leader:

We investigated the role of fimbrial and afimbrial adhesions in the attachment of Xylella fastidiosa (Xf) to grape. We have individually disrupted FimA, FimF, XadA, and HxfB to assess their role in adhesion to plants and in the disease process. We performed adhesion assays using each mutant and wild-type separately as well as a combination of two mutants at one time to observe the phenotypes of...

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Mapping analysis of the Xylella fastidiosa genome.

A cosmid library was made of the 2.7 Mb genome of the Gram-negative plant pathogenic bacterium Xylella fastidiosa and analysed by hybridisation mapping. Clones taken from the library as well as genomic restriction fragments of rarely cutting enzymes were used as probes. The latter served as a backbone for ordering the initial map contigs and thus facilitated gap closure. Also, the co-linearity ...

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ژورنال

عنوان ژورنال: Microbiology

سال: 2010

ISSN: 1350-0872,1465-2080

DOI: 10.1099/mic.0.037564-0